Molecular cloning of a gene encoding the histamine H2 receptor.
نویسندگان
چکیده
منابع مشابه
Molecular cloning of the human histamine H2 receptor.
We utilized the technique of polymerase chain reaction with oligonucleotide primers based upon the nucleotide sequence of the canine H2 histamine receptor gene which we recently isolated to clone its human homologue. Transfection of a construct of this gene in Colo-320 DM cells led to the expression of a receptor that bound to [methyl-3H] tiotidine and was linked to 3',5'cyclic adenosine monoph...
متن کاملCloning and molecular characterization of TaERF6, a gene encoding a bread wheat ethylene response factor
Ethylene response factor proteins are important for regulating gene expression under different stresses. Different isoforms for ERF have previously isolated from bread wheat (Triticum aestivum L.) and related genera and called from TaERF1 to TaERF5. We isolated, cloned and molecular characterized a novel one based on TdERF1, an isoform in durum wheat (Tri...
متن کامل[Structural analysis of the histamine H2 receptor].
In gastric parietal cells the histamine H2 receptor is involved in gastric acid secretion. Molecular cloning of the cDNA for the histamine H2 receptor has revealed that the receptor is a member of the G-protein coupled receptor family. We expressed histamine H2 receptors in CHO cells, COS7 cells, and Sf9 cells to analyze N-glycosylation, palmitoylation, and desensitization of the H2 receptor, w...
متن کاملHistamine H2 receptor trafficking: role of arrestin, dynamin, and clathrin in histamine H2 receptor internalization.
Agonist-induced internalization of G protein-coupled receptors (GPCRs) has been implicated in receptor desensitization, resensitization, and down-regulation. In the present study, we sought to establish whether the histamine H2 receptor (H2r) agonist amthamine, besides promoting receptor desensitization, induced H2r internalization. We further studied the mechanisms involved and its potential r...
متن کاملCloning of the gene encoding the human erythropoietin receptor.
The genomic and complementary DNAs of the human erythropoietin receptor (hEpo-R) have been isolated and characterized from a genomic placental library and from two cDNA libraries prepared from bone marrow and fetal liver. The five different partial cDNAs isolated were aberrant in the predicted reading frames as compared with the Epo-R protein sequence, because all retained insert sequences that...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1991
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.88.13.5937a